Projects
Stem cell differentiation and disease model
I established neuronal differentiation protocol from patient derived iPSC lines, using different commercial kits:
validated with end point assays such as RT-qPCR, IF, Bulk RNA Seq, Single cell RNA Seq.
Supported in myeloid progenitor, striatal spheroids and neuromuscular organoids differentiation from control and patient
derived iPSC lines to study neuro-degenerative disease models. Supported in optimization of cardiomyocytes differentiation
from human iPSC lines to study cardio-vascular disease models.
Correction of point mutation in patient iPSC lines using CRISPR Cas9 engineering system;
validated using digital PCR, followed by single cell plating, colony picking, reconfirmation, cryopreservation.
Varicella virus (VZV) based study
Grew, maintained, and tittered various viruses such as VZV GFP, VZV WT, HSV1, EBV, Zika via plaque assays ensuring laboratory efficiency and adherence to GLP/GMP standards. Used non-viral vector based SiRNA mediated gene silencing techniques in stem cell derived neurons.
Studied Varicella Zoster Virus infection, latency, and reactivation in both hESC based neurons and iPSC derived Sensory Neurons. Analyzed RNA and protein samples via FISH, RT-PCR and western blot.
Neuronal injury Model Study
Grew, maintained, and tittered various viruses such as VZV GFP, VZV WT, HSV1, EBV, Zika via plaque assays ensuring laboratory efficiency and adherence to GLP/GMP standards. Used non-viral vector based SiRNA mediated gene silencing techniques in stem cell derived neurons.
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Micro-fluidic Device co-culture system for Neuroinflammation Model study
Executed projects involving axonal injury and regeneration, microglia interaction in CNS based injury, a nerve-bone cell interaction study that were performed in micro fluidic devices.
Microglia phagocytosis of degenerating axons that involved TLR/TRIF pathway using neurons (mice & rats) and microglia were studied using microfluidic devices.
Axonal-cell body protein analysis were performed via western blot and immunostain.
Neuro-protective drug/compound Screening studies
At Johns Hopkins, Involved in stem cell-based studies to analyze TBI and MS related drug screening. Used luminescence-based assay to screen high throughput axon protective drugs such as Curcumin, Flecainide, Paroxetine, Kv 1.3 along with MAPK inhibitors to stop wallerian degeneration. Studied nanoparticles-based drug delivery system in astrocytes using Flow Cytometry.
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Nanoparticle based drug delivery/Injury model
Worked under team of Dr. Jeff Winkles, Dr. Anthony Kim and Dr. Graeme Woodworth, who are working as a “Translational Therapeutics Research Group” to solve life-limiting treatment challenges in neuroscience and cancers with the help of nanomaterial.
Conducted optimization of nanoparticle with cytokines delivery to minimize non-specific binding, increase tissue penetration, and dispersion in rat pups model.
Conducted study of mechanical stress in astrocytes grown in matrigel based 3D culture at cellular level, a Traumatic Brain Injury Model.